Search results for " sperm"

showing 10 items of 127 documents

Circulating progesterone levels and ongoing pregnancy rates in controlled ovarian stimulation cycles for in vitro fertilization: analysis of over 400…

2010

background: The influence of elevated serum progesterone levels during in vitro fertilization/intracytoplasmic sperm injection (IVF/ ICSI) cycles on pregnancy rates is a matter of continued debate among fertility clinicians. Efforts to resolve this question have been impeded by the various assays used to measure progesterone and the different, arbitrary threshold values for defining ‘high’ progesterone levels. methods: A non-interventional, retrospective, observational, single-centre cohort study evaluated the relationship between serum progesterone levels on the day of human chorionic gonadotrophin (hCG) administration and the ongoing pregnancy rate in 4032 patients undergoing IVF/ICSI cyc…

Adultmedicine.medical_specialtyPregnancy Ratemedicine.drug_classmedia_common.quotation_subjectmedicine.medical_treatmentFertilityStimulationFertilization in VitroGonadotropin-releasing hormoneBiologyChorionic GonadotropinIntracytoplasmic sperm injectionCohort StudiesGonadotropin-Releasing HormoneAndrologyOvulation InductionPregnancyInternal medicinemedicineHumansProgesteroneRetrospective Studiesmedia_commonPregnancyIn vitro fertilisationbusiness.industryRehabilitationObstetrics and GynecologyGeneral MedicineOdds ratioMiddle Agedmedicine.diseaseConfidence intervalPregnancy rateEndocrinologyReproductive MedicineRegression AnalysisReproductive Control AgentsGestationFemaleOvulation inductionFollicle Stimulating HormoneGonadotropinbusinesshormones hormone substitutes and hormone antagonistsHormoneHuman Reproduction
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The heparin-glutathione test: an alternative to the hypo-osmotic swelling test to select viable sperm for intracytoplasmic sperm injection

1998

Abstract Objective: To evaluate the heparin-glutathione test (HEGLUT) for the selection of viable sperm for intracytoplasmic sperm injection (ICSI). Design: A prospective study. Setting: Department of Pediatrics, Obstetrics and Gynecology, University of Valencia and Instituto Valenciano de Infertilidad. Patient(s): Semen samples from healthy donors and patients with infertility. Intervention(s): Sperm samples were kept in culture for different periods in Ham's F-10 medium supplemented or not supplemented with heparin, reduced glutathione (GSH), or a heparin-GSH mixture. Control and heparin-GSH–treated spermatozoa were injected into hamster oocytes. The HEGLUT and ICSI were performed. Main O…

Infertilityurogenital systemmedicine.medical_treatmentObstetrics and GynecologyHamsterSemenBiologyAsthenozoospermiamedicine.diseaseOocyteSpermIntracytoplasmic sperm injectionAndrologymedicine.anatomical_structureReproductive Medicinemedicinereproductive and urinary physiologySperm motilityFertility and Sterility
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Induced hyperactivity in boar spermatozoa and its evaluation by computer-assisted sperm analysis.

2004

Hyperactivity, a form of sperm motility characterized by vigorous flagellar movements, has been proposed as essential for fertilization in mammals. The objective of the present study was to establish a method for inducing hyperactivityin vitroin boar spermatozoa and to define threshold values to differentiate between hyperactive and non-hyperactive spermatozoa by computer-assisted sperm analysis (CASA) as a prerequisite for analyzing the energy metabolism during hyperactivity. In TALP-HEPES medium, non-frozen boar spermatozoa were stimulated to hyperactivity by 50 μmol l−1Ca2+within 15 min at 37 °C if 5 μmol l−1of the Ca2+ionophore A23187 was present. If 25% seminal plasma was present, boar…

Maleendocrine systemEmbryologyBOARSwineEnergy metabolismCell Culture TechniquesSemenBiologyAndrologyEndocrinologyHuman fertilizationSemenImage Interpretation Computer-AssistedAnimalsBoar spermSperm motilityCalcimycinIonophoresSperm Counturogenital systemCurve analysisObstetrics and GynecologyCell BiologyAnatomySpermSpermatozoaReproductive MedicineSperm MotilityCalciumEnergy MetabolismReproduction (Cambridge, England)
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Sperm DNA integrity and human papillomavirus (HPV) infections: a controversy that could be resolved by a new molecular approach

2020

Study question: The aim was to determine if HPVs affect spermatozoa DNA integrity. To resolve the discrepancy regarding the association between HPV infections and sperm DNA damage. Summary answer: To elucidate if HPV impairs DNA integrity, we suggest to investigate HPV DNA positivity in spermatozoa by a differential lysis procedure before TUNEL assay.

HPV SpermatozoaSettore MED/07 - Microbiologia E Microbiologia ClinicaSettore BIO/11 - Biologia MolecolareSettore BIO/06 - Anatomia Comparata E CitologiaTUNEL
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Molecular Alterations in Spermatozoa of a Family Case Living in the Land of Fires. A First Look at Possible Transgenerational Effects of Pollutants.

2020

In our previous work, we reported alterations in protamines/histones ratio, in DNA binding of these proteins and their involvement in DNA oxidative damage in 84% of the young men living in the Land of Fires. In the present work, we extended our findings, evaluating any alterations in spermatozoa of a family case, a father and son, living in this area, to also give a first look at the possibility of transgenerational inherited effects of environmental contaminants on the molecular alterations of sperm nuclear basic proteins (SNBP), DNA and semen parameters. In the father and son, we found a diverse excess of copper and chromium in the semen, different alterations in SNBP content and low DNA …

0301 basic medicineMaleProtamineprotein-DNA binding010501 environmental sciences01 natural sciencesAntioxidantslcsh:ChemistryOxidative damageHistoneschemistry.chemical_compoundProtaminesSettore BIO/06 - Anatomia Comparata E CitologiaLand of Firesheavy metalslcsh:QH301-705.5SpectroscopyGeneticsbiologySperm CountSperm Motility Nuclear Proteinhuman protaminestransgenerational effectsHuman protamineNuclear ProteinsGeneral MedicineMiddle AgedSpermatozoaComputer Science ApplicationsDNA oxidative damageHistoneHeavy metalSperm MotilityEnvironmental PollutantsAntioxidantAdolescentDNA damageSemenEMSACatalysisArticleInorganic Chemistry03 medical and health sciencesTransgenerational epigeneticsSemenHumansPhysical and Theoretical ChemistryMolecular BiologyEnvironmental PollutantLand of FireInfertility Male0105 earth and related environmental sciencesPollutantOrganic ChemistryTransgenerational effectEnvironmental ExposureHydrogen PeroxideProtaminehuman spermatozoaSemen Analysis030104 developmental biologyFertilitylcsh:Biology (General)lcsh:QD1-999chemistrybiology.proteinDNADNA DamageInternational journal of molecular sciences
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Antioxidants in ICSI

2013

Male factor infertility can be caused by reasons, either related or not with total sperm production. Among causes of male infertility in cases of normal sperm count and motility, oxidative stress is one of the most relevant processes influencing fertility in vivo or in assisted reproduction treatments’ results. This chapter provides the most updated information regarding the oxidative stress situation in sperm and the relevance of antioxidants use in intracytoplasmic sperm injection results.

endocrine systemurogenital systembusiness.industrymedia_common.quotation_subjectmedicine.medical_treatmentFertilitymedicine.diseasemedicine.disease_causeSpermIntracytoplasmic sperm injectionMale infertilityAndrologyMedicineDNA fragmentationReproductionbusinessSpermatogenesisreproductive and urinary physiologyOxidative stressmedia_common
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Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertiliz…

2005

To determine the prognostic value of sperm DNA fragmentation levels, as measured by the sperm chromatin dispersion (SCD) test, in predicting IVF and ICSI outcome.Double-blind prospective study.University-affiliated private IVF setting.A total of 85 couples undergoing infertility treatment with IVF/ICSI.Analysis of DNA fragmentation by the SCD test in 170 aliquots obtained from the ejaculate and from the processed semen used for assisted reproductive technologies (ART).Percentage of spermatozoa with fragmented DNA was statistically correlated with embryo quality and reproductive success.Fertilization rate was inversely correlated with DNA fragmentation (r = -0.245 P = .045). Higher DNA fragm…

AdultMaleZygotemedicine.medical_treatmentEmbryonic DevelopmentReproductive technologyDNA FragmentationFertilization in VitroBiologyIntracytoplasmic sperm injectionAndrologyPredictive Value of TestsPregnancymedicineHumansEmbryo ImplantationSperm Injections IntracytoplasmicFragmentation (cell biology)reproductive and urinary physiologyIn vitro fertilisationPronucleusurogenital systemObstetrics and GynecologySpermSpermatozoaChromatinBlastocystTreatment OutcomeReproductive MedicineFertilizationembryonic structuresDNA fragmentationFemaleEmbryo qualityCell NucleolusFertility and sterility
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Closed vs. Open Oocyte Vitrification Methods Are Equally Effective for Blastocyst Embryo Transfers: Prospective Study from a Sibling Oocyte Donation …

2020

<b><i>Purpose:</i></b> To assess whether open and closed vitrification protocols are equally effective for sibling-oocyte cycles when performing blastocyst embryo transfers. <b><i>Materials and Methods:</i></b> A prospective study was set up comparing the open and the closed vitrification techniques in oocyte recipients sharing sibling oocytes between 2014 and 2016. Sibling oocytes were randomly and equally assigned into the closed group (oocytes vitrified in a closed system) or the open group (oocytes vitrified in an open system). Intracytoplasmic sperm injection was performed on all cases. Embryo transfers were performed on day 5. Power anal…

AdultPregnancy Ratemedicine.medical_treatmentIntracytoplasmic sperm injectionAndrology03 medical and health sciences0302 clinical medicineHuman fertilizationPregnancymedicineHumansVitrificationBlastocystAdult Birth RateEmbryo ImplantationProspective StudiesSperm Injections IntracytoplasmicBirth RateSurvival rateCryopreservation030219 obstetrics & reproductive medicineOocyte Donationbusiness.industryObstetrics and GynecologyEmbryoOocyteEmbryo TransferVitrificationInfertility; Oocyte donation; Vitrificationmedicine.anatomical_structureBlastocystReproductive Medicine030220 oncology & carcinogenesisInfertilityOocytesFemaleLive birthbusinessGynecologic and obstetric investigation
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Abnormalities caused by chloroform and ether in the spermatogenesis of mammals [Chloroforma un ētera radītie traucējumi zīdītāju spermatogenezē]

1937

Teksts angļu valodā, kopsavilkums latviešu valodā.

Zoology experimentalAnimal experimentsPeles - eksperimentiDzīvnieku eksperimentiZīdītāju spermatogeneze - eksperimenti:NATURAL SCIENCES::Biology::Organism biology::Morphology [Research Subject Categories]Mammalian spermatogenesis - experimentsZooloģija eksperimentālā
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Kinetics of the early development of uniparental human haploid embryos

2016

Objective: To describe morphokinetically the early development of human haploid parthenotes and androgenotes and to compare them with euploid embryos. Design: Experimental study of kinetics. Setting: University-affiliated private fertility center. Patient(s): Experimental haploid parthenotes and androgenotes. Intervention(s): Kinetic study of early development (up to eight cells) of 8 parthenotes, 10 androgenotes, and 20 euploid embryos. Main Outcome Measure(s): Timing of the first seven cleavages determined according to embryo origin, then calculation of the duration of the second and third cell cycles (cc2 and cc3) of whole embryos and individual cells. Result(s): Parthenotes and androgen…

0301 basic medicineAdultanimal structuresAdolescentmedicine.medical_treatmentEmbryonic DevelopmentBiologyHaploidyIntracytoplasmic sperm injectionAndrology03 medical and health sciencesYoung Adult0302 clinical medicinemedicineHumansSperm Injections IntracytoplasmichumanGenetics030219 obstetrics & reproductive medicineOutcome measuresObstetrics and GynecologyEmbryoOocyte activationAndrogenoteAneuploidyKineticshaploid030104 developmental biologyReproductive Medicinemorphokineticparthenoteembryonic structuresPloidy
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